The influence of discoidin domain receptor 1 expression on angiogenic factors: VEGF-A and FGF-2 in non-small cell lung cancer

Lung cancer is responsible for more cancer-related death worldwide. Most lung patients have non-small cell cancers (NSCLC), with a low survival rate. Therefore, it necessary to study new and effective targeted therapies. Discoidin domain receptor 1 (DDR1) tyrosine kinase that can be promoted by collagen type I, dysregulation of DDR1 signaling involved in several phases tumorigenesis. Tumor growth metastasis depend on angiogenesis caused angiogenic factors, such as vascular endothelial factor (VEGF) fibroblast (FGF) secreted tumor cells. Thus, plays critical role progression. The current amid evaluate the expression alteration its effects factors VEGF-A, FGF-1, FGF2 NSCLC. A549 Calu-3 cells were treated I transfected small interfering RNA (siRNA). Then, relative DDR1, FGF-2 was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). concentration calculated Human Angiogenesis 17-plex discovery assay. According our data, could stimulate cells, upregulation resulted significant increase VEGF-A expression, but did not induce FGF-1 expression. Moreover, suppression significantly decrease findings suggest mediated siRNA may affect associated represent treatment approach.